The field of molecular biology continues to be the most exciting and dynamic area of
science and is predicted to dominate the 21st century. Only at the molecular level, it
is possible to understand various biological processes in detail. Such understanding
is vital for advances in medicine, and the pharmaceutical industry that produces new
drugs and thus cure is greatly dependent upon molecular biology. Molecular
biology also contributes to our understanding of what human beings are and how
they fit into this universe.
The objective of this manual is to apprise the teachers and students at B.Sc. & M.Sc.
levels about the various techniques in molecular biology. This book contains four
chapters giving the step-wise protocols for various practicals. In addition, it contains
general instructions for safety procedures, viz., biosafety, radiation safety, hazardous
chemicals, personal protection and also the preparation of various stocks and
working solutions for molecular biology practicals.
S.K.Gakhar is a Professor of Biotechnology, Maharshi Dayanand University, Rohtak.
At present he is also holding the charge of Director, Centre for Bioinformatics and
Centre for Medical Biotechnology. He is associated with teaching of Molecular
Biology for the last 25 years at Post-graduate level and engaged in research in the
field of malaria parasite & mosquito vector interactions. He has in total 71 research
publications till date and has successfully completed 11 major research projects. He
has supervised near about 12 Ph.D. thesis. He was awarded with Young Scientist
Award in the year 1990. He is also working as Cordinator of DST – FIST, UCG – SAP
and Bioinformatics Centre. He is also program coordinator of Interdisciplinary Life
Science Program (IPLS).
Monika Miglani is working in the Centre for Biotechnology, Maharshi Dayanand
University, Rohtak. She is engaged in research in the field of Insect Genetic
Engineering. She has submitted novel sequences in the GenBank database. She has
published papers nationally and internationally. She has also worked as “Course
Instructor” in Short Term Training Course on Molecular Biology organized by C.B.T,
M.D.U. sponsored by D.B.T, Govt. of India.
Ashwani Kumar is working in the Centre for Biotechnology, Maharshi Dayanand
University, Rohtak. He is engaged in research in the field of Insect Genetic
Engineering. He has also worked as “Course Instructor” in Short Term Training
Course on Molecular Biology organized by C.B.T, M.D.U. sponsored by D.B.T, Govt.